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In humans with more than one X chromosome, the number of Barr bodies visible at interphase is always one fewer than the total number of X chromosomes.

For example, men with Klinefelter syndrome (47, XXY karyotype) have a single Barr body, whereas women with a 47, XXX karyotype have two Barr bodies.

Shown is a single optical section made with a confocal microscope.

Bottom: Same nucleus stained with DAPI and recorded with a CCD camera.

rendered inactive in a process called lyonization, in those species in which sex is determined by the presence of the Y (including humans) or W chromosome rather than the diploidy of the X.